earticle

영어

The β-carotene 15,15'-monooxygenase, cleaves β-carotene into two molecules of retinal, catalyzes the first step in vitamin A synthesis. The β-carotene 15,15'-monooxygenase gene from Mus musculus was cloned into pET 24(a) vector and then expressed Escherichia coli. The expressed enzyme was purified by His-taq affinity and resource Q union exchange column chromatographies with a final specific activity of 0.505 mU/mg. The enzymatic activity of β-carotene 15,15'-monooxygenase to produce retinal from β-carotene was optimal at pH 9.0 and temperature 37 ℃.