원문정보
초록
영어
The gene coding for carboxymethylcellulase (CMCase) of B. amyoliquefaciens DL-3 was isolated and cloned in E. coli JM109F. Rice bran and tryptone were chosen as carbon and nitrogen sources for production of CMCase by E. coli JM109F, respectively. Optimal conditions such as concentrations of carbon sources and nitrogen sources, initial pH of medium, and temperature for production of CMCase were investigated using L25(5)3 - orthogonal array method
with five distinct levels and three factors. The highest production of CMCase was 1,314.2 U/ml from 5.0% (w/v) rice bran and 0.1% (w/v) tryptone under initial pH of 7.5 and 37℃. Optimal conditions of salts for production of CMCase were also investigated using L25(5)4. The highest production of CMCase E. coli JM109F was 1,514.6 U/ml with 0.5% (w/v) K2HPO4, 0.025% (w/v) NaCl, 0.04% MgSO4․7H2O and 0.24%(w/v) (NH4)2SO4.