원문정보
초록
영어
Thioredoxin-1 (TRX) is a redox-active protein with multiple intracellular and extracellular functions1-2).TRX has recently been shown to be responsible for the onset of various diseases. However, at present, the mechanism and pathway by which extraceullular TRX influences cellular behavior remain unknown3-4). The aim of this study was to determine the binding partner of exogenous TRX. We first found that exogenous TRX (tagging with hexa-histidine) internalized into cell. We also used His-TRX pull down experiment, immunoprecipitation,
LC-MS/MS, and ESI Q-TOF MS/MS to identify membrane proteins involved in the entrance of TRX. As a result, several membrane proteins including GRP78 were found to bind exogenous TRX, which may play a crucial role in TRX internalization and signal transduction. Our studies are in progress to understand the molecular mechanisms of the TRX entry under the inflammatory response and oxidative stress.