Mussel adhesive protein (MAP) fp151-RGD designed and constructed by our research laboratory
has excellent adhesion and spreading abilities Based on its adhesive and basic properties, fp-151-RGD is possibly suitable for use as a cell-adhesion material in cell culture or tissue engineering, and in any other area where efficient cell adhesion is required. In this aspect, we designed oppositely charged polyelectrolyte multilayer (PEM) of fp151-RGD (+) and hyaluronic acid (HA) (-) as a platform surface to improve complication of titanium-based implantation such as aseptic loosening. Each layer was deposited by spin coater, and the built-up of both fp151-RGD and HA layer were confirmed by quartz crystal microbalance (QCM) and contact angle analyzer. Then, adhesion of pre-osteoblast cells including MC3T3-E1 was examined on the multilayer films by direct cell count and MTT methods. As a result, adhesion properties of the cells were superior in the surface-treated titanium to pure titanium foils. Our results suggest that the method of PEM using fp151-RGD and HA can be applied as an efficient titanium-based implant.