원문정보
초록
영어
DNA amplification, by polymerase chain reaction(PCR), are widely used in molecular biotechnology for various purpose. New DNA amplification method, loop-mediated isothermal amplification method(LAMP), was developed to solve the problems of the conventional PCR such as repeated thermal cycles and interferences from impurity DNAs [1-2]. The LAMP process is performed at a constant temperature (between 60℃-65℃), providing DNA amplification of 109-1010 times in about 1 hour. Two identical primer sequences are designed in
a single strand so that they can form a terminal loop. By this way, high-temperature denaturation could be avoided. We applied the LAMP method to EGF(epidermal growth factor) and HPV(human papilloma virus) genes. When the amplified products were digested with a restriction enzyme(AluI) and monitored by turbidity from magnesium pyrophosphate precipitation [3], the
results confirmed that both genes are successfully amplified. Compared with the conventional PCR method, LAMP method was superior in terms of: shorten reaction time(3-4 times) with improved efficiency. It can be used for forensic applications in particular when a gene in a minute amount of complex specimens should be amplified rapidly.