원문정보
초록
영어
Lipase B from Candida antarctica (CalB) is an efficient biocatalyst for many organic synthesis reactions. To expand the application of CalB, many researchers have used directed evolution or rational approach to create CalB mutants.
Especially thermostable enzymes are usually required to enable the production process at high temperature. We have already reported the synthesis of glycerol carbonate using CalB.
Reaction at higher temperature about 80℃ can make this process favorable, because higher concentration of substrates can be converted due to the increase of solubility. Rational approach was selected to develop thermostable CalB. As the first step, the choice of the appropriate sites at which rigidity sets a high value was made on the basis of atomic displacement parameters obtained from X-ray data. And then the sites are changed with other amino acids which are
more rigid than original amino acids. As a result of rational approach, several thermostable CalB mutants had been found. In addtion, CalB mutants were successfully expressed in the methylotropic yeast P ichia pastoris and the Escherichia coli cytoplasm.