원문정보
초록
영어
The Escherichia coli strains expressing either NADH-dependent or NADPH-dependent azoreductase (EC 1.6.5.2), mediating reduction of azo-dye, were cultured in the glucose minimal medium with presence of 200 μM methyl red for the introduction a specific reducing poser perturbation of either NADH or NADPH. The growth rates during the perturbation of NADH-perturbed and NADPH-perturbed cells were 0.05 h-1 and 0.12 h-1, respectively, when that of control cell was 0.13-1. The glucose consumption for biomass formation of NADH-perturbed cells was 10.8 g-glucose/g-cell while those of control and NADPH-perturbed cells showed little variation (3.6 VS 3.8 g-glucose/g-cell) during perturbation phase.
The greater influence of NADH-perturbation than NADPH on the cellular growth is discussed. (KOSEF R01-2007-000-20231-0)