원문정보
초록
영어
Exogenous fatty acids and its derivatives influence various cellular processes. In Escherichia coli,
a long-chain acyl-CoA is a regulatory signal that modulates gene expression and it bindsdirectly
to the transcription factor FadR, thus preventing transcriptional activation and repression of many
genes. In this study, comparative proteomic analysis of E. coliin the presence of glucose and
oleic acid was performed to understand cell physiology in response to oleic acid. Among total of
52 proteins showing altered expression levels with oleic acid presence, 9 proteins including AldA,
Cdd, FadA, FadB, FadL, MalE, RbsB, Udp and YccU were newly synthesized. Among thegenes
that were induced by oleic acid, the promoter of the aldA gene was used for the production of
a green fluorescent protein (GFP). Analysis of fluorescence intensities and confocal microscopic
imagesrevealed that soluble GFP was highly expressed under the control of the aldA promoter.
These results suggest that proteomic study is playing an important role not only in biological
research but also in various biotechnological applications. [This work was supported by the
Korean Systems Biology Research Grant from the Ministry of Education, Science and Technology
through the Korea Science and Engineering Foundation (No. M10309020000-03B5002-00000).
Further supports by LG Chem Chair Professorship, IBM SUR program, Microsoft, and by the
KOSEF through the Center for Ultramicrochemical Process Systems are appreciated.].