원문정보
초록
영어
Coenzyme A (CoA) is a ubiquitous essential cofactor. CoA is the source of 4` -phosphopantetheine, the prosthetic group of carrier proteins of fatty acid, polyketide and nonribosomal peptide synthases(1). Pantothenate kinase (PanK) is the key regulatory enzyme in the CoA biosynthetic pathway(2). Most polyketides are synthesized by the assembly of precursors such as acethyl-, propionyl- and butyl-CoA. Their polyketide synthesis precursors are activated derivatives, malonyl-, methylmalnoly- and ethylmalonyl-CoA. Recently, the pantothenate kinase gene cluster was analyzed from S. peucetius genome project. The
multiple amino acid sequence of SpPanK shows 52% and 58% similarity with the comparison of EcPanK and M tPanK. We cloned panK gene from S. peucetius ATCC 27952 and constructed the expression vector in pET32a. The expressed protein is 36 kDa. The enzyme assay was monitored to ADP formation by coupling reactions with pyruvate kinase and lactate dehydrogenase. The activity was measured as a change in absorbance at 340 nm during
monitoring of depletion of NADH(3).