원문정보
초록
영어
Our purpose on this study is bacteriocins identification by secretome analysis through plasmid curing-encoding antimicrobial peptide.
We isolated Lactobacillus strain showing antimicrobial activity against Staphylococcus aureus from Kimchi. To identify a bacteriocin and analyze secretomes from Lactobacillus spp.. Plasmid curing was performed using chemical agents such as ethidium (EtBr), novobiocin and sodium dodecyl sulfate (SDS). Plasmid curing was verified by comparing plasmid profile of wild type and cured type on 0.7 % agarose gel and colorimetric assay to detect bacteriocin production. Bacteriocin identification and secretome analysis were carried out by comparative mass spectrometry using 2D-LC profiling and De novo sequencing.
This work was supported by a grant (20080401034009) from BioGreen 21 program, Rural
Development Administration, Republic of Korea.