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This study was designed to examine the antioxidant activities of the essential oil and various extracts (hexane, chloroform, ethyl acetate and methanol) of Cestrum nocturnum L. Further, the total phenolic contents of the essential oil and extracts were determined, which was expressed as garlic acid equivalents (GAE). Ethyl acetate extract contained higher phenolic content (12.36 GAE mg/g) than other extracts. The antioxidative potential of the samples was evaluated by scavenging capacities of 1,1-diphenyl-2-picrylhydrazyl (DPPH) and superoxide radicals. The extracts and essential oil displayed remarkable scavenging capacities of DPPH (IC50 range of 42.56-49.85 μg/ml) and superoxide radical (IC50 range of 18.37-19.26 μg/ml). Our findings demonstrate that the essential oil and various extracts of C. nocturnum possessed antioxidant
activities that might be a natural potential source of preservative used in food and other allied industries.