원문정보
초록
영어
Chitooligosaccharides (COSs) are derivative of chitosan and it can be obtained by either enzymatic or chemical hydrolysis of chitosan. COSs are not only water-soluble but also possess versatile biological activities such as antitumor, immunostimulating, antioxidant and antimicrobial activity1). This study investigated antioxidative effects of carboxymethyl chitooligosaccharides (CM-COS) on the generation of direct free radical scavenging activity by using electron spin resonance (ESR) spectrometer and measure Fourier transform infrared (FT-IR) spectroscopy. Antioxidative activities of CM-COS at 70% and 80% were measured, respectively. And cytotoxic effect was measured by the method of Park et al1).
In the results of ESR spectrometer, the CM-COS 80% had the highest antioxidant activity compared to the other CM-COS. The IC50 values of 80% CM-COS were 3.29, 6.25, 2.65 and 1.50 mg/ml for DPPH, hydroxly, superoxide, alkyl radical, respectively. Moreover, CM-COS did not show any cytotoxic effect against MRC-5 cell lines.