원문정보
초록
영어
The functional Lipase B from Candida antarctica(CalB) was expressed in a cell-free protein synthesis system derived from Escherichia coli . While most of the cell-free synthesized CalB was insoluble under conventional reaction conditions, the combined use of molecular chaperones led to the soluble expression of CalB. Inaddition, the functional enzyme was generated by applying the optimal redox potential. When examined using pNPP as a substrate, the specific activity of the cell-free synthesized CalB was higher than that of the reference protein produced in Pichia pastoris. These results highlight the potential of cell-free protein synthesis technology as a powerful platform for the rapid expression, screening and analysis of industrially important enzymes.