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Candida antarctica lipase B(CALB) is an important catalyst in bio-organic synthesis. CALB was successfully expressed in the yeast P ichia pastoris and Saccaromyces cerevisiea. However, the use of E.coli for the production of CALB has faced many practical and biological problems such as insoluble aggregates and proteolytic degradation. In this research, for the enhancement expression of CALB in E.coli , CALB was expressed in periplasm using PelB signal sequence.
And the research of chaperon-CALB fusion was performed to improve the expression level and solubility of CALB in E. coli .
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자료제공 : 네이버학술정보