원문정보
초록
영어
The production of biodiesel by enzymatic transesterification has attracted much attention for
high purity product and enables easy separation. Candida antarctica lipase B is one of the
most important enzyme in the biodiesel reaction. In reaction, CALB shows low organic
solvent stability while having relation with methanol. In this study, we concerned about
enhancing organic solvent stability in hydrophilic solvent. In water-miscible solvents, water
molecule seems to play an important role in biological structure and function. Water
molecules in the enzyme are stripped away or replaced with hydrophilic solvent molecules
and this causes deformation and denaturation of the enzyme. From the directed evolution
studies in hydrophilic solvents, introduction of polar or charged amino acids increased
hydrogen bonds and increased enzyme stability. Increase of hydrogen bondinteraction can
make water molecule less strips off and more stable in hydrophilic organic solvents.
Hydrogen bond interaction can be increased by shorten the distance of amino acids and water
molecule and also increase electrostatic interaction. ASP and ASN was replaced to GLU and
GLN of CALB for in silico mutagenesis. Mutants were analyzed by hydrogen bonding
network and solvent accessible surface area.