원문정보
초록
영어
Aptamers are nucleic acid species that have been engineered through repeated rounds, SELEX
(systematic evolution of ligands by exponential enrichment) to bind to various molecular targets.
They offer the utility for biotechnological and therapeutic applications as they have molecular
recognition properties that rival that of the commonly used biomolecule, antibodies.
Carcinoembyonic antigen(CEA) is a glycoprotein involved in cell adhesion. It was found that
serum from individuals with colorectal, gastric, pancreatic, lung and breast carcinomas had
higher levels of CEA than healthy individuals. CEA measurement is mainly used as a tumor
marker to identify recurrences after surgical resection. First, CEA has bond on the magnetic
bead through chemical bonding and it was confirmed by CEA specific antibody(ELISA).
Second, We synthesized a labeled(fluorescence) single strand DNA(ssDNA) for CEA, using
asymmetric PCR with a 5’-labeled primer. Finally, through the repeated rounds of SELEX,
specific ssDNA was recovered and verified by its binding affinity.