원문정보
초록
영어
The electrochemical regeneration of nicotinamide cofactor is of particular interest in
oxidoreductase catalyzed reactions such as chemical synthesis, degradation of pollutant,
construction of biosensor, and fuel generation. Because most of oxidoreductase enzymes
require expensive cofactors for catalysis, the cofactors have to be regenerated. For the
efficient cofactor regeneration under low overpotential, indirect methods utilizing mediators
have been frequently used. Reactions using mediators can occurred under low overpotential
and prevent electrode fouling, but some of these mediators are toxic to the enzymes. Also, the
stability of the mediator can affect the whole reaction performance. Therefore, construction of
mediator-free cofactor regeneration system is very attractive and necessary. In this study, we
screened several kinds of new metal oxides as an electrode material for direct electrochemical
cofactor regeneration. Using these new metal oxide electrode, cofactors were regenerated
efficiently under low overpotential without using mediator. Details will be presented and
discussed.