원문정보
초록
영어
Lipases are used as efficient biocatalysts in many industrial processes. Especially, Candida antarctica lipase B (Cal B) draws attention because it can be used for stereoselective transformations and polymer synthesis, and the potential process for biodiesel production using lipase instead of chemical catalysts is under development. Cal B was expressed in the methylotrophic yeast P ichia pastoris X-33 using pPICZαB vector, which has α-factor, helping protein secretion by methanol induction. The colonies showing a halo were selected, and
the expression and secretion of Cal B in flask cultivation with glycerol as a carbon source and methanol induction was confirmed by SDS-PAGE and the activity test using p-NPP. A 5-l fermentor was used to investigate the effects of cultivation conditions on the lipase expression and secretion.