원문정보
초록
영어
Saccharomyces cerevisiae is one of the several well-known microorganisms, the importance of which in bio-ethanol production is increasing. The aim of this study was to investigate the efficiency of commercially available enzyme, Spirizyme Plus FG (glucoamylase), for enzymatic saccharification of food waste. Subsequently, the ethanol fermentation of the obtained hydrolyzates was performed by S. cerevisiae KA4, which was previously isolated in the three-stage metane fermentation system developed in this lab. The saccharification conditions such as substrate concentration, enzyme activity, reaction time, pH and temperature were optimized. The obtained optimal conditions were as follows: substrate concrntration, 80 g COD/L; enzyme
concentration, 400 AGU/L; reaction time, 4h; pH, 7 and temperature, 55oC. Under the optimal condition, 105 g/L of reducing sugar was produced from 80 g/L of food waste. This hydrolyzates was subjected to the ethanol fermentation under anaerobic condition described previously using S. cerevisiae KA4. Finally, KA4 was able to produce 65 g/L of ethanol during 24h at 35oC.