원문정보
초록
영어
Mononuclear aromatic hydrocarbons such as benzene, toluene, ethylbenzene, and xylene (BTEX) are widespread as soiland groundwater contaminants. We examined the anaerobic degradation of individual BTEX components by soil bacteria with nitrate, sulfate or iron as electron acceptors. Biodegradation of all BTEX components was successfully achieved with all electron acceptors. Toluene and xylene were rapidly degraded than ethylbenzene followed by benzene with much slower rate. Terminal restriction fragment polymorphism(TRFLP) analysis
of soil samples adapted for over one-year for microbial community structure showed high diversity with benzene or xylene as substrates that were strongly dependent on available electron acceptors. Identical anaerobic cultures grown on either toluene or ethylbenzene showed two diverse microbial community clusters, each dependent on substrate specificity irrespective of electron acceptors. Variability of microbial community structure within these two clusters was
identified with only sulfate as electron acceptor but not with nitrate or iron. Our results suggests that it is possible to select one or more substrate-electron acceptor partners for effective bioremediation of mixed BTEX components in simulated or natural environments.