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논문검색

Analysis of microbial communities during anaerobic biodegradation of xylene under different electron-accepting conditions

초록

영어

During anaerobic degradation of aromatic compounds, electron acceptors play major role. In this study, degradation of xylene, which has the lowest degradation rate among BTEX, with nitrate, sulfate, or iron as electron acceptors were investigated. By adding electron acceptor, degradation rate of xylene was increased. Specifically, degradation rate of xylene was significantly increased under nitrate reducing condition. In order to investigate microbial community structures during anaerobic degradation of xylene under different electron acceptors, DGGE (denaturing gradient gel electrophoresis) analysis of the PCR amplified 16S rRNA gene fragments adapted for one year old soli samples. Microbial community structures were simplified under nitrate, sulfate, and iron reducing conditions compared to those in the
condition without electron acceptor. It suggest that microorganisms which degrade xylene efficiently were dominated under each reducing conditions. Based on the phylogenetic analysis of the DGGE bands, it was identified that the consortium consisted of four spirochaetes, two betaproteobacteria, two deltaproteobacteria, and one gemmatimonadetes. The most abundant band was closely related with the bacterium which were found in petroleum and
hydrocarbon contaminated sites.

저자정보

  • Sun-Mi Lee Center for Environmental Technology Research, Korea Institute of Science and Technology
  • Ji-Seong Kim Center for Environmental Technology Research, Korea Institute of Science and Technology
  • Byoung-In Sang Center for Environmental Technology Research, Korea Institute of Science and Technology

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