원문정보
초록
영어
A major cause of diabetes is impaired insulin action in adipose tissue, skeletal muscle and liver. Among the tissue, the liver is the largest organ in the body, has a numerous molecules such as carbohydrates, amino acid, lipids, plasma protein, drug and xenobiotics. Diabetes mellitus (DM) is a chronic progressive disease that often results in microvascular and macrovascular complications, yet its pathogenesis in liver of diabetic patients is not clear. Previously study, They findings of redox changes in the eye and aorta of diabetic rats, as well as identified differential expression of proteins in kidney, eye, aorta, and serum of diabetic and non diabetic rat. In the present study, we have tried to explain the effect of diabetes in the liver. And we have sought to characterize the protein differently expressed in rat liver using 2-dimensional
electrophoresis. For making hyperglycemia enviroment, diabetes of rats were induced by injection of STZ. At the 3th and 10th week after STZ injection, liver sample of STZ-treated rat were prepared and analyzed by IEF and SDS-PAGE. After image analysis, several proteins induced by hyperglycemia in liver of rats were identified.