원문정보
초록
영어
Nanotoxicology has attracted attention due to the growing use of nanoparticle in the diagnostic and therapeutic biomedical application. Here, we present in vitro cytotoxicity screening of magnetic nanoparticles which are widely employed as contrast-enhancement agents for magnetic resonance imaging (MRI). The water dispersible MnO and Fe3O4 nanoparticles encapsulated by polyethyleneglycol (PEG)-phospholipid or PEG-derivatized phosphine oxide were synthesized and their toxicity to the various human cell lines (A549: Lung cancer cell, MCF7: Breast cancer cell, SK-N-SH: Neuroblastoma cell) were evaluated. In addition, the cytotoxic effect of concentration of nanoparticles and incubation time were also studied using three kinds of fluorescence-based assays (Reactive oxygen species (ROS), Lactate dehydrogenase (LDH), and Live/Dead viability kit).