원문정보
초록
영어
At present, cell-based drug screening assays are employed for various molecular interactions studies including induction of apoptosis. Screening of apoptosis would allow both early detection of therapy efficiency and evaluation of disease progression [1]. In this study, the affinity of between annexin V and phosphatidylserine (PS) was confirmed by using surface plasmon resonance (SPR). An early apoptotic marker, annexin V was used for quantification of MCF-7 cells' apoptosis to determine drug efficacy of staurosporine (SSP) as an anticancer agent [2]. Propidium iodide (PI) was used to differentiate necrotic from apoptotic cells. Cellular imaging obtained by confocal microscopy was processed by ROI (region of interest) setting up
of cell boundary, and its fluorescence intensity was quantified to compare it with the MTS assay results, which is one of the standard assays for cell viability. Quantitative determination of apoptotic cells using annexin V-FITC was proportional to the cell viability results from MTS assay. Therefore, this study presents a feasibility of using cellular imaging technique as a means to quantitatively determine cell viability.