원문정보
초록
영어
Diabetic nephropathy (DN) is the most common cause for end stage renal disease (ESRD). Proteomics and metabolomics offer a nonbiased suite of tools to address pathophysiologic mechanisms and discovery of biomarkers associated to disease. In this study, serum protein expressions in diabetic patients without complications and patients who have microalbuminurea were compared using serial cut-off to enrich low-molecular-weight proteome. Three spots were
up-regulated about 100-200% in microalbuminuric patients compared to normoalbuminuric patients. Among these differentially expressed spots apolipoprotein A-Ⅳ was identified with ESI-Q-TOF mass spectrometry.
Secondly, peptides whose molecular weight less than 10 kD were analysed with reversed-phase high performance liquid chromatography (RP-HPLC). Fractions showing different % peak area were collected and peptides were analyzed by N-terminal sequencing. Eight amino acid residues were detected and the peptide was identified as potassium channel, subfamily K, member 6 variant. Finally, filtrates of 3 kD cut-off membrane were analyzed by nuclear magnetic
resonance (NMR) apparatus to search metabolites affected with diabetic nephropathy.