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논문검색

Expression and N-glycan Pattern Analysis for Human 90K Glycoprotein in Drosophila S2 Cells

초록

영어

Cancer that is one of serious diseases for human causes angiogenesis, metastasis, and
abnormal cell divisions including genetic change. Thus, lots of researchers have been
investigated to inhibit cancer. Human 90K (h90K) protein has very crucial role for inhibition of cancer metastasis. h90K is a glycoprotein having potential 7 N-glycosylation sites that 4 sites are clear but remaining 3 sites are not yet. In this work, we performed expression of recombinant h90K glycoprotein in Drosophila S2 cell system. We constructed stablytransfected S2-h90K cells by antibiotic selection and confirmed h90K expression using Western Blot and in vitro bioactivity analyses. We also identified N-glycan patterns of recombinant h90K glycoprotein using HPLC and MALDI-TOF MS. Therefore, this research is going to development of engineered glycosylation for humanized recombinant 90K in Drosophila S2 cells.

저자정보

  • Kyoung Ro Kim National Research Laboratory of Molecular Biotechnology and Department of Chemical Engineering, Pohang University of Science and Technology
  • Yeon Kyu Kim National Research Laboratory of Molecular Biotechnology and Department of Chemical Engineering, Pohang University of Science and Technology
  • Dong Gyun Kang National Research Laboratory of Molecular Biotechnology and Department of Chemical Engineering, Pohang University of Science and Technology
  • So Young Jang Mass Spectrometry Research Team, Korea Basic Science Institute
  • Young Hwan Kim Mass Spectrometry Research Team, Korea Basic Science Institute
  • Hyung Joon Cha National Research Laboratory of Molecular Biotechnology and Department of Chemical Engineering, Pohang University of Science and Technology, Corresponding Author

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