earticle

영어

Most of thermal or chemical processes for pretreating biomass are carried out at an extreme
condition, and the generation of undesirable byproducts such as inhibitors for enzymatic and
fermentation is unavoidable. The biological pretreatment using white-rot basidiomycetous fungi could offer potential improvement in the current pretreatment and deconstruction of biomass by minimizing inhibitors and energy consumptions. In the present study, a lignindegrading enzyme was selected as targets for the primary tool for disabling the natural recalcitrance of lingnocellulsic biomass. The production of a fungal lignin-degrading enzyme, manganese peroxidase (MnP) by Phanerochaete chrisosporium and Dichomitus squalens was optimized for achieving the maximal pretreatment effect. Culture conditions such as carbon, nitrogen, trace minerals, and vitamin sources were optimized against the activity of MnP in both cell-envelop-bound and intact-cell-bound forms by the response surface methodology (RSM) including the Plackett-Burman design (PBD), Box-Behnken design (BBD) and Ridge Analysis (RA). For the direct measurement of change in the enzyme digestibility, cellulose hydrolysis enzymes of Trichoderma reesei cellulase and Aspersillus niger beta-glucosidase were used. The fungal-pretreated rice straw was tested for ethanol production in the process of simultaneous and saccharification using cellulase and Saccharomyces cerevisiae.