원문정보
초록
영어
Poly(lactic-co-glycolide) (PLGA) microspheres have been investigated for a long time as a protein/peptide delivery depot, and some products were already commercialized. However, recent publications have report about their disadvantages in the long term delivery of protein/peptide drugs such as the denaturation, aggregation, and deamidation of protein/peptide drug. The phenomena are induced by proton generated from the hydrolysis of ester linkages in PLGA. It is well known that an acidic condition facilitates the denaturation, aggregation, and deamidation. Therefore, the development of new protein/peptide delivery depot is necessary. In this study, pullulan acetate microsphere (PAM) was inspected as a depot for long term delivery of exendin-4 which is a drug for type II diabetes. It was prepared by w/o/w double emulsion method. The mean particle size of PAM ranged from 5 to 100 mm, as determined by SEM. Their shapes were regular and spherical, as visualized via SEM photographs. The exendin-4 loading efficiency of the microspheres was up to 90 wt. %. The microspheres may give an idea for
development of new protein/peptide depot in long term delivery.