원문정보
초록
영어
Erythropoietin (EPO) is a glycoprotein hormone primarily synthesized in kidney and regulates mammalian erythropoiesis1). In this study, N-glycans of recombinant human erythropoietins (rhEPO) expressed in insect cells were analyzed to investigate the heterogeneity according to different cell culture methods. rhEPO was designed to have mouse derived IgM signal sequence for secretion out of the cell and IgG binding domain of protein A at N-terminal for efficient purification. Although insect cells are usually cultured at 27℃, various culture temperatures such as 24℃, 30℃, 33℃, 37℃ were tested. 24℃ showed highest productivity for rhEPO and the productivity was decreased according as the culture temperature increased. Where as the productivity was increased at low temperature, N-glycan profile of rhEPO from 24℃ culture was similar with that of 27℃ culture.