earticle

영어

Mesenchymal stem cells (MSCs) hold great promise in cell-based therapies because of their multipotency and simple methods for in vitro expansion. However, during in vitro expansion, MSCs undergo aging and loss their multipotency and proliferation capability. Previous studies have reported that calorie restriction (CR) increases proliferation of MSCs and decreases
apoptosis(1). Therefore, we examined the effect of low glucose in MSC in vitro.
Proliferation in the low glucose (LG, 1.375 mM) condition was compared with that in the normal glucose (NG, 5.5 mM) condition. In addition, comparative studies of population doubling, β-galactosidase activity, ROS generation and differentiation capacity (osteocyte, adipocyte) in NG and LG conditions were performed. We compared protein expressions between NG and LG conditions, several proteins were up-regulated or down-regulated in glucose restriction
condition, LG condition. Although a large-scale study is necessary to confirm the clinical function of the proteins elucidated in this study, these results imply that differentially-expressed proteins in LG condition may provide further information on the aging and differentiation of stem cells.