원문정보
초록
영어
Mesenchymal stem cells (MSC) exhibit finite proliferative potential in vitro, the so-called Hayflick limit. According to the free-radical theory, reactive oxygen species (ROS) may be potential candidates responsible for senescence and age-related diseases, and on production of high levels of ROS, the redox balance is disturbed and cells shift into a state of oxidative stress, which subsequently leads to premature senescence with shortening telomeres. H2O2 has been the most commonly used inducer of stress-induced premature senescence (SIPS), which shares features of replicative senescence (RS): similar morphology, senescence-associated β-galactosidase activity, cell cycle regulation, etc.
Therefore, we confirmed the senescence of hMSC during SIPS by various analysis. And we performed proteome profiling to find regulators associated with senescence. Then we confirmed that the proteins we found are equally regulated in RS.