원문정보
초록
영어
Increased levels of reactive oxygen species (ROS) can damage various cellular processes. We examine the overall and nanoscale morphologies and mechanics of cytostructural elements of human skin cells during oxidative stress-induced skin damage. Human skin fibroblasts exposed to oxidizing agents were imaged with atomic force microscopy (AFM), and proteomic tools were used to clarify ROS signaling-responsive proteins. The cytoskeletal organization, morphology,
oxidative stress, and viability were also marked at the following time points.
Oxidative agents induced time- and concentration- dependent disruption of native organization of cytoskeleton with a decrease in cell integrity. These dynamic changes in cell mechanical elements were accompanied by roughness increase, morphological changes, increased oxidative stress, and loss of cell viability. A water-soluble derivative of vitamin E, Trolox, showed a preventive effect on the ROS-induced cellular damage. The results indicate that alterations of cellular constituents seem to activate the cell death cascade/apoptosis-related
proteins. Furthermore, the oxidative damage to cytoskeleton might occur at relatively early stages of exposure.