원문정보
초록
영어
Recent developments of effective skin-whitening agents have focused on melnogenesis inhibition followed by decreasing total melanin contents. Keratinocyte secretes stimulator of melanogenesis to neighboring melanocytes, providing protection against UV radiation. Keratinocytes also produce other cytokines, such as IL-1α, IL-6, TNF-α and TGF-β, which inhibits melanogenesis.1) In this study, inhibitory melanogenesis was investigated in
melanoma cells treated with keratinocytes-cultured medium contains Angelica gigas extract. To decide the proper treatment concentration of extract, MTT assay was performed with Keratinocytes. Keratinocytes-cultured medium was collected 24h later, and immediately used to culture melanoma cells. 2D PAGE analysis was done for investigation of melanogenesis related proteins. Acknowledgements
This research work was supported by a grant from the Engineering Research Center for Advanced Bioseparation Technology, KOSEF.