원문정보
초록
영어
Background/purpose: Recently, many reports have shown the potential of new materials as cosmetic ingredients. These have demonstrated beneficial effects on skin recovery and wound healing. In this study, we investigated the effects of fermented rice bran extracts on damaged skin cells. In this study, we have focused on the possible effects of rice bran extracts on UV-irradiated normal human skin keratinocytes.
Method: Normal human skin was aseptically isolated from a circumcised neonatal foreskin. And epidermal layers were mechanically stripped and keratinocytes were isolated form the epidermis. The human keratinocytes were seeded at a density of 1ⅹ105 cells/well in 6 well plates and then irradiated after 1 day. Prior to irradiation, cells were washed with PBS and irradiation was performed through a thin film of PBS via exposure to a 20 mJ/㎠ with UV B of 312 nm dose, as measured with an SX-312 research radiometer. Human normal keratinocytes were UV irradiated and treated 0.1%, 0.5% and 1% of FRBE and 25nM ascorbic acid.
Results: Cell viability following UV irradiation was significantly higher in the groups treated with FRBE at doses of 0.1, 0.5, and 1.0%, in comparison to the control group with UV irradiation only. And UV-irradiated cells demonstrated decreased levels of laminin as well as increased cytokine production (MMP-1, MMP-2), relative to nonirradiated controls.
Conclusion: We found that treatment of these UV-irradiated keratinocyte with fermented rice bran extracts increased laminin protein levels and decreased cytokine production. In conclusion, the data obtained in this work suggest that fermented rice bran extract has potential as a medical treatment following UV exposure.