원문정보
초록
영어
Isoflavones, one of the major groups of phytoestrogens, have anticarcinogenic, antioxidant and photoprotective effects. Although they have beneficial bioactivities for human health, lower bioavailability and poor solubility have been problems in applications. To address this problems, we first have optimized large-scale conditions for enzymatic bio-conversion of crude
isoflavone glucosides from soy into aglucones by using a β−glucosidase.
Further purification processes enabled us to obtain more than 90 % purity of isoflavone analyzed by HPLC. The purified isoflavone showed to a have strong anti-inflammation activity. UV irradiation of HaCaT cells induced TNF-α production by 5 folds but the treatment of the purified isoflavone reduced TNF-α production back to normal level. In order to make derivatives
of isoflavone, which might have novel bioactivities with better solubility, acylation reactions of the purified isoflavone aglucones were first performed by using a lipase from P sedomonas cepacea. However, the conversion rate was no more than 50 % even after 7 days of reaction at 50 ℃. On the other hand, acylation reactions of crude isoflavone glucosides showed higher
conversion rate than the purified isoflavone aglucones. More than 50% of acylated glucoside isoflavone derivatives were formed in 3 days. Further optimization of the reaction is being carried out.