earticle

영어

Basic characteristics of astaxanthin and H aematococcus pluvialis cracked cell, including solubility and stability were investigated, and the extraction conditions were optimized for the astaxanthin production from H . pluvialis cracked cells. Acetone was selected as the best solvent for the extraction of astaxanthin from H . pluvialis. Response surface methodology (RSM), based on a two-factor interaction model, was applied, and optimization of the extraction parameters
such as β-glucanase concentration and sonication time was performed. Astaxanthin yields was the highest when it was extracted after H . pluvialis cell was treated by β-g lucanase(2%) and the sonication w as carried out spontaneously with the extraction for 75 min. Additionally, inclusion complexes of astaxanthin with various cyclodextrins were prepared and characterized with respect to water-solubility and stability. The best host material for the inclusion of astaxanthin was selected as β-cyclodextrin. The optimum ratio of astaxanthin and cyclodextrin for the inclusion complex formation was found to be 1 : 200. As a results, the solubility of the inclusion complex (As-β-CDIC) was about 110 times higher than free astaxanthin at pH 6.5 and 25℃, respectively.