원문정보
초록
영어
Aromatic nitro groups are found in diverse types of important antibiotics, but the biosynthesis is poorly understood. Spectinabilin with antiviral and antimalarial activities is one of nitrophenyl-containing compounds. Here, we cloned and characterized a spectinabilin biosynthesis gene cluster of 45.4 kb from Streptomyces spectabilis and heterologously produced spectinabilin in Streptomyces lividans. Fourteen genes including type I modular polyketide synthases are expected to be related to spectinabilin biosynthesis. Remarkably, an iterative modular type I polyketide synthase is involved in the biosynthesis of spectinabilin. An ADC lyase with p-aminobenzoate N-oxygenase and pABA synthase catalyzes the generation of a rare p-nitrobenzoate start unit. An additional bimodular polyketide synthase catalyzes two additional successive chain extensions, compared to aureothin cluster. The results provide insights of biosynthetic pathways of other spectinabilin derivatives.