원문정보
초록
영어
It is very critical to develop the surface plasmon resonance (SPR) detection in order to achieve an avian influenza (AI) virus sensor. This work is specifically focused on the development of biosensor system to obtain recombinant proteins with the gold-specific affinity. The strategy is based on the principle that the gold binding polypeptide (GBP) as a fusion partner specifically binds onto the gold surface for immobilizing proteins. The AI virus surface antigen was employed as a model protein for the specific immobilization of GBP-fusion protein. The fusion proteins were successfully immobilized on the gold surface and examined by SPR and SPR imaging analysis. We also analyzed the immobilization efficiency of intracellular protein on the micro-patterned gold surface.
Finally, the GBP-fusion method immobilized proteins onto the gold surface without surface modification and in a bioactive form, which is suitable for studying interactions of protein-protein, and other biomolecules [This work was supported in part by the IT Leading R&D Support Project from the Ministry of Knowledge Economy through IITA].