earticle

영어

The kinetic parameters for Salmonella typhimurium binding with the immunoassays were evaluated quantitatively. The lower limit of detection for the cell was also investigated in the immunoassays using a surface plasmon resonance biosensor. The lower limit of detection for the cell was improved to 101 CFU/ml by application of the sandwich assay. The association rate constants and affinities of the cell by the sandwich assay were above twice as large as those by the direct assay. The improvement of the lower limit of detection by the sandwich assay was derived from the enhancement of binding attraction between the cell and a detection antibody.