원문정보
초록
영어
In this study, we have developed shear horizontal (SH) surface acoustic wave (SAW) sensors for
the detection of immunoglobulin G (IgG) [1] on the gold coated delay line of SH-SAW devices.
The sensor consists of twin SAW delay lines operating at 100MHz, one reference channel and one
sensing channel, fabricated with Au/Ti on 36YX LiTaO3piezoelectricsubstrate.The delay line was
exposed to a solution of gold nanocatalyst-anti-mouse IgG(G-anti MIgG) conjugate with in PBS
solution. Target mouse IgG solution was injected onto the delay line. As the result of the
experiment, we could uniformly immobilize anti-MIgG(mouse IgG) conjugate on the surface of gold.
When displaying results of immobilization on the surface of gold using G-anti MIgG conjugate and
blocking buffer in frequency shift, G-anti MIgG conjugate showed frequency shift of 75.1 kHz in
the initial frequency, and blocking buffer showed frequency shift of 215.7 kHz. When various
concentration of MIgG was added in 100MHz type, the sensor showed 46.3, 127.45, 161.21 and
262.39 kHz frequency shift at 25, 50, 75 and 100 μg MIgG concentration compare with phosphate
buffered saline (PBS) solution. The result shows that is primarily detected the frequency shift due
to the change in mass loading, which results from interactions between the prove and the target
layer.