earticle

영어

Bio-butanol has become one of the possible alternatives to gasoline fuel. Its better haracteristics such as higher energy content, less hydroscopic and volatile which make it possible to use pre-existing transport infrastructure, than ethanol is regaining the reputation of conventional ABE (Acetone-Butanol-Ethanol) fermentation. However, the potent toxicity of butanol on the main ABE producers derived from Clostridium acetobutylicum ATCC 824, Clostridium beijerinckii NCIMB 8052, Clostridium saccharoperbutylacetonicum N1-4 etc., is still a main drawback of commercialization as a fuel. Extensive studies have been done only to reach the best titer around 19.2 g/L. In this study, we developed new HTS (High-Throughput-Screening) systems specific to butanol based on designed enzymatic reaction. Along with HTS system, we designed a programmed chemical mutagenesis system suitable for screening strains resistant to membrane toxicity. Using our original HTS system coupled with programmed random mutagenesis, we successfully developed a mutant strain, Clostridium beijerinckii CBP-381, which produced at least 30% more butanol under the optimized fermentation condition.