원문정보
초록
영어
Basic biological and medical research continues to provide the building blocks necessary to
develop solutions to human health problems and to preserve and repair the natural environment.
In this study, we investigated the feasibility of applying a RI detection technique to a protein
kinase C assay for the development of a diagnostic biochip. Using the mep45 gene encoding
the 45 kDa major envelope protein (Mep45) of Selenomonas ruminantium, a Mep45-fused
substrate was cloned and purified for use as substrate to detect the activity of protein kinase C.
The resultant MFS-PKC protein was used as a substrate for a protein kinase C assay and the
signal intensity of the kinase assay system was examined with varying concentrations of the
kinase. The results are found to be promising and can be applied when using a RI detection
technique for developing a highly sensitive biochip to determine the enzyme activity for a
disease diagnosis and subsequent new drug development.
Keywords: Protein kinase C, Protein-fused substrate, Microarrays, Radioisotope