원문정보
초록
영어
Cell cycle-dependent protein kinases (CDKs) regulates the cells entry into mitosis during the
transition from G2 to M phase, by phosphorylating a group of key proteins including RNA
polymerase II and histone H1. A recombinant substrate for the enzyme was expressed as a
recombinant protein from a plasmid pMFS-cdc2 in E. coli, and it was purified. In this study,
we present a strategy for the detection of a phosphorylation of a substrate catalysed by cdc2
protein kinase by using a radioisotope detection technique in microarrays. We conclude that
the use of Mep45-fused substrate in developing a protein biochip for the detection of the activity
of cdc2 protein kinase by using a RI detection technique is highly economical and can be used
for both high throughput screening and studying protein-protein interactions.
Keywords: Cdc2 protein kinase, Gene cloning, Microarrays, Radioisotope detection