원문정보
초록
영어
Single stranded DNA aptamers that bind to tetracycline (TET) with high affinity and specificity were identified by selection from an oligonucleotide library of 1015molecules using modified SELEX, which was employing tosylactivated magnetic beads. The binding affinities of seven aptamers are in nanomolar range. These DNA aptamers are highly selective for TET as much as distinguishable of only single hydroxyl group on 5 or 6 carbon atoms in other tetracycline group. TET binding DNA aptamer, No.20 (Kd=63.6nM) was used for the electrochemical detection of TET. To fabricate electrochemical aptasensor, No.20 DNA aptamer was immobilized on screen-printed gold electrode chip on which all of three working, reference and counter electrode were
integrated. After treatment of sample containing chemicals, cyclic voltammetry and squire wave voltammetry were analyzed. In result, the electrochemical aptasensor showed a good sensitivity in a range of 1-10000nM and the selectivity of this aptasensor for TET detection was also verified with other antibiotics of tetracyclines' group.