원문정보
초록
영어
The optimal fed-batch process for high cell density cultivation of E. coli XL1-Blue/pHCE-InaN-GAPDH-Ghost 37 was evaluated. The nutrient feeding strategy with Riesenberg defined medium during fed-batch process was determined. The fermentation was conducted in four phases. The
initial phase(phase 1) was the batch growth phase. Phase 2 was a fed-batch phase with an exponential feeding rate to control the specific cell growth rate to 0.15 h-1. Phase 3 was the phase of temperature increase to 42℃ for the induction of E lysis gene for the ghost cell formation. In this phase, the specific growth rate was reduced to 0.03 h-1. Phase 4 was high
temperature holding phase at 47℃ for 2 h to increase ghost cell efficiency.
At the end of the cell-lysis process, efficiency of the bacterial ghost formation in non-lyophilized E. coli/ GAPDH antigen was 99.7% with temperature induction at OD600 = 50. The maximum bacterial ghost vaccine was obtained about 22 g/L. And the protective efficacy of ghost cell with
antigen GAPDH was determined by the challenge test to immunized olive flounder with live S. iniae. Therefore, the ghost E. coli / GAPDH antigen from S. iniae could be used as effective and commercially available vaccine in aquaculture for the prevention of streptococcal disease.