원문정보
초록
영어
In this study, we investigated the population dynamics and biokinetic evaluation of ammonia oxidizing bacteria(AOB) in a laboratory-scale bioreactor. The bioreactor was operated from batch to continuous stage and its substrate was the industrial wastewater including 300 mg/L ammonium nitrogen and 6 mg/L total cyanides which is known as toxic chemicals for AOB. The AOB communities were identified through molecular analysis by 16S rRNA DGGE/PCR and quantified by real-time PCR. The primer-probe sets, group specific sets for the detection of β-proteobacterial AOB, were used in order to compare the AOB population dynamics at the level of species. Nitorosomonas europaea, N. nitrosa and N. aestuarii were identified at full-scale process of industrial wastewater as seed for the reactor. In addition, three AOB clusters that are NSMeur, NSMnit and NSMcry were quantified and NSMnit and NSMcry were major AOB cluster at batch system especially. NSMcry was rapidly dropped and only NSMnit survived as a major cluster while the batch process was shifted into the continuous mode. We applied the Monod equation to the evaluation of AOB biokinetic parameters. The values of μmax, Ks, Y and
kd were 0.434 d-1, 10 mg NH4 +-N/L, 0.564 mg MLVSS/mg NH4 +-N and 0.057 d-1 based on volatile suspended solid respectively.