원문정보
초록
영어
Microbial community of ammonia-oxidizingbacteria (AOB) in a full-scale activated sludge process for a period of one year was investigated using real-time PCR quantification (QPCR) and denaturing gradient gel electrophoresis (DGGE). Because the wastewater treatment plant is located on coastal area, seawater often infiltrates into the plant due to heavy rain including Typhoon.
Such inclusion of seawaters is likely to result in high concentration of chlorides, sodium, and sulfates in wastewater treatment plant. In this research, concentration of salts in the influent was five times higher than the domestic sewer. Composition of the influent mixed with the infiltrate was also very similar to that of average seawater. To understand the AOB diversity, 16S
rRNA-gene-targeted QPCR and DGGE was performed with group-specific primer and probe sets. AOB belonging to Nitrosomonas cryotolerans cluster (NSMcry) were dominant in all samples and the population ranged from 7.8 x 105 to 2.0 x 107 cells/mL. AOB belonging to the cluster of N. europaea (NSMeur) were also detected and the cell concentration ranged from 5.6 x 103 to
9.9 x 104 cells/mL. Total number of AOB community was 0.3-3.4 % of the total bacterial population. The DGGE profiles of the two group-specific primers showed AOB belonging to each group that were not detected in the DGGE profile of the universal primer set, CTO. According to the identification of DGGE band, haloterants and halophilic AOB were dominant in this process such as N. halophila, N. cryotolerans, N. marina, and N. eutropha.