earticle

영어

We present the electrochemical detection of DNA mutation on peptide nucleic acid (PNA)-based electrode utilizing single strand specific enzyme, S1 nuclease. Electrochemical detection of DNA mutation is based on the change of surface charge caused by hybridization of negatively charged DNA molecules with the complementary or non-complementary binding to PNA probe. When target DNA is perfectly matched with PNA, the DNA/PNA duplex is not digested by the
nuclease, while DNA/PNA duplex including one-base mismatch is hydrolyzed by nuclease, meaning that negatively charged mediator leads to charge repulsion when negatively charged DNA is hybridized with PNA. On the other hand, this mediator can easily approach the neutral PNA-modified electrode surface without target resulting from removing by target DNA due to S1 nuclease. With enzymatic cleavage by S1 nuclease, we successfully demonstrated its clinical
utility by detecting mutation DNA. Therefore, a mismatched target DNA could be discriminated from a perfectly matched DNA.