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Application of low temperature to a CHO cell culture for improving recombinant protein production and quality

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Lowering culture temperature has become popular in a CHO cell culture for improving recombinant protein production and quality. Many reports showed that a reduction in culture
temperature (28°C-32°C) resulted in growth arrest, metabolism decrease and improved cell
viability with variable effects on recombinant protein productivity. Most published studies
revealed lowering culture temperature enhanced specific productivity1, qp, while a few
demonstrated no increase in qp at low culture temperature2. Recently, it has been shown that
enhancing effect of low culture temperature on qp depends on clones3. Therefore, the selection
of clones displaying enhanced qp at low culture temperature is critical for improvement of
recombinant protein production. In addition to enhanced qp, lowering culture temperature is known to be advantageous for improving protein quality4 and reducing protein aggregation5. Owing to its various beneficial effects, the application of low culture temperature to a CHO cell culture will be more attractive.

저자정보

  • Sung Kwan Yoon Bio R&D Center, Genexel-Sein

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