earticle

영어

The technique of serological analysis of antigens by recombinant cDNA expression library (SEREX) uses autologous patient sera as a screening probe to isolate tumor-associated antigens for various tumor types. Isolation of tumor-associated antigens that are specifically
reactive with patient sera, but not with normal sera, is important to avoid false-positive and
autoimmunogenic antigens for the cancer immunotherapy. Here we describe a selection
methodology to isolate patient sera-specific antigens from yeast surface-expressed cDNA
library constructed from 15 patient lung tissues with non-small cell lung cancer (NSCLC).
Several rounds of positive selection using patient sera alone as a screening probe isolated
clones exhibiting comparable reactivity with both patient and normal sera. However, the
combination of negative selection with allogeneic normal sera to remove antigens reactive
with normal sera and subsequent positive selection with patient sera efficiently enriched
patient sera-specific antigens. Using the selection methodology described here, we isolated
3 known and 5 unknown proteins, which have not been isolated previously, but potentially
associated with NSCLC.